Wollscheid, Bernd, Dr.

Dr. Bernd Wollscheid and his research group are focusing on the development of a qualitative and quantitative understanding of the cellular surfaceome as a complex signaling gateway to the microenvironment. Further information about our research topics can be found here: Google Scholar / ResearchGate / PubMed

News

May 2013

ETH Zurich licensed our TRICEPS-based LRC technology to Dualsystems Biotech AG, Schlieren, Switzerland. An LRC kit and LRC service will become available commercially soon.

Our collaborative biomedical project together with Jean-Pierre Bourquin's group from the Children's Hospital entitled "Leukemia surfaceome analysis reveals new disease-associated features" published in Blood. Congrats to Andreas and Paulina!

Dr. Andreas Frei was awarded with the ETH Zurich Medal for his outstanding PhD thesis. Congrats Andy!

April 2013

The detailed LRC protocol entitled "Ligand-based receptor identification on living cells and tissues using TRICEPS" was just accepted by Nature Protocols and will be available online within a couple of weeks. Congrats to Andy, Karel and Hansjoerg!

March 2013

"Malfunctioning of adipocytes in obesity is linked to quantitative surfaceome changes" manuscript just accepted for publication in Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids. Congrats to Hansjoerg Moest who finished this project as an interdisciplinary PhD student inbetween the Wollscheid and Wolfrum laboratories.

Sebastian Mueller was awarded with a travel fellowship from the Molecular Life Sciences PhD program (MLS) of the Life Science Zurich Graduate school supporting his recent attendance of the Proteomics Forum 2013 of the German Society of Proteome Research in Berlin. Congrats!

The lab says goodbye to Dr. Hansjoerg Moest who joined the pharmaceutical company Sanofi-Aventis. Best of luck!

February 2013

The lab says goodbye to Dr. Ferdinando Cerciello who joined the Cancer Center of Ohio State University as a Postdoctoral fellow supported by an SNF Medical fellowship. Best of luck!

Ulrich Omasits and Nadine Sobotzki were awarded with DGPF-Travel-Fellowships supporting their attendance of the upcoming Proteomics Forum 2013 of the German Society of Proteome Research in Berlin. Congrats!

A team effort enabled the generation of a new resource for the biomedical proteomics community: "N-Glycoprotein SRMAtlas: a resource of mass-spectrometric assays for N-glycosites enabling consistent and multiplexed protein quantification for clinical applications" manuscript just published in Molecular & Cellular Proteomics. Congrats to everybody involved!

A collaborative host-pathogen study led by Lilli Stergiou from the Pelkmans lab entitled Integrin-Mediated Signaling Induced by Simian Virus 40 Leads to Transient Uncoupling of Cortical Actin and the Plasma Membrane was accepted in the journal PLOS One. Congrats to Lilli who recently joined Redbiotec.

January 2013

The Wollscheid lab contributed to a project led by Paola Picotti from the Aebersold lab entitled "A complete mass-spectrometric map of the yeast proteome applied to quantitative trait analysis" just published in Nature. Congrats Paola!

The lab says goodbye to Dr. Andreas Frei who joined Gary Nolan's group in Stanford as a Postdoctoral fellow supported by an SNF fellowship. Best of luck!

The Wollscheid lab was awarded with a grant from the Swiss Commission for Technology and Innovation (CTI) supporting the commercialisation and future development of the TRICEPS-based LRC technology.

December 2012

Paulina Mirkowska defended her PhD thesis entitled "Chemoproteomic Cell Surface Mapping for the Detection of Disease Associated Features in Childhood Acute Lymphoblastic Leukemia". Congratulations!

Nikiana Simigdala defended her PhD thesis entitled "The Drosophila Wing Disc Proteotranscriptome at Spatial Resolution". Congratulations!

Nature Chemical Biology picked the LRC technology as a "Chemoproteomics research highlight" in an article entitled: Flex your TRICEPS

November 2012

The ETH Zürich Foundation has awarded the Wollscheid lab with a grant for cross-disciplinary research in translational and personalised medicine.

The Wollscheid lab was rewarded with an Agilent technologies "University Research Program" grant supporting the development of innovative chemoproteomic workflows.

Nature Methods picked the LRC technology as a "Chemical Biology" highlight in an article entitled: The Power of TRICEPS

Ferdinando Cerciello successfully defended his MD PhD thesis entitled "Proteomics approaches for Malignant Pleural Mesothelioma". Congratulations!

Review entitled "Proteomics approaches for the analysis of enriched microbial subpopulations and visualization of complex functional information" published in Current Opinion in Biotechnology

October 2012

Nature Biotechnology "News and Views" article by Sarah A Slavoff & Alan Saghatelian entitled "Discovering ligand-receptor interactions" provides an interesting perspective about the LRC technology and TRICEPS.

ETH Life article entitled "Trifunctional molecule decodes cellular communication" highlights the LRC technology, TRICEPS and its applications.

September 2012

"Direct identification of ligand-receptor interactions on living cells and tissues" manuscript published in Nature Biotechnology.

Our TRICEPS patent entitled "TRIFUNCTIONAL CROSSLINKING REAGENTS" is now accessible online

We welcome Emanuela Milani, who started her PostDoc in our group funded by a Roche fellowship.

Damaris Bausch-Fluck joined our team again after her maternity leave. Welcome back!

August 2012

"Proteomic analysis reveals drug accessible cell surface N-glycoproteins of primary and established Glioblastoma cell lines" manuscript just accepted for publication in Journal of Proteome Research.

"Cell Surface Capturing (CSC) technologies for the surfaceome discovery of hepatocytes" manuscript published in Methods in Molecular Biology: Liver Proteomics

Review entitled "Teneurin protein family: An emerging role in human tumorigenesis and drug resistance" published in Cancer Letters.

Perspective article entitled "Innovative proteomische Strategien zur Erkennung von neuen Blutbiomarkern" published in Leading Opinions Hämatologie & Onkologie.

July 2012

"The stalk domain and the glycosylation status of the activating natural killer cell receptor NKp30 are important for ligand binding" manuscript accepted for publication in Journal of Biological Chemistry

Ulrich Omasits won a European Conference on Computational Biology 2012 (ECCB’12) Conference Fellowship award for his abstract entitled "Protter - an open-source protein feature visualization software"

Hansjoerg Moest successfully defended his PhD thesis entitled "Cell surface proteins in the adipose tissue". Congratulations!

Karel Novy's abstract entitled " Quantitative interrogation of host protein phosphorylation networks modulated by Vaccinia infection" was selected for oral presentation at HUPO 2012 in Boston.

Ferdinando Cerciello's abstract entitled " Glycopeptide Serum Markers for Malignant Pleural Mesothelioma Diagnostics" was selected for oral presentation at HUPO 2012 in Boston.

"RIP-chip-SRM--a new combinatorial large-scale approach identifies a set of translationally regulated bantam/miR-58 targets in C. elegans" manuscript published in Genome Research

June 2012

Nadine Sobotzki won a HUPO 2012 (Boston) travel stipend for her abstract entitled "TRICEPS For Drug-Based Receptor Capturing (DRC)".

Andreas Frei successfully defended his PhD thesis entitled "A chemoproteomic technology for ligand-based receptor identification". Congratulations!

Andrea Jacobs joined the new lab of Bernd Bodenmiller at the University of Zuerich as lab manager/technician focusing on mass-cytometry/CYTOF research.

Ferdinando Cerciello won the poster award at the 3rd symposium of the Swiss Clinical Trial Organization (SCTO) at the University Hospital Zurich focusing on "Personalised Medicine in Clinical Research".

"Identification and functional characterization of pVHL-dependent cell surface proteins in renal cell carcinoma" manuscript published in Neoplasia

May 2012

"CSC Technology - Selective Labeling of Glycoproteins by Mild Oxidation to Phenotype Cells" manuscript accepted for publication in Methods in Molecular Biology: Mass Spectrometry of Glycoproteins

Dynamin 2 mutations in Charcot-Marie-Tooth neuropathy highlight the importance of clathrin-mediated endocytosis in myelination" manuscript published in Brain

April 2012

Paulina Mirkowska's abstract entitled "Discovery and validation of new stratification markers in childhood acute lymphoblastic leukemia" was selected for oral presentation at the 17th Congress of the European Hematology Association (EHA) in Amsterdam.

Ferdinando Cerciello's abstract entitled " Glycopeptide Serum Markers for Malignant Pleural Mesothelioma Diagnostics" was selected for oral presentation at the 3rd European Lung Cancer Conference (ELCC) in Amsterdam.

Andreas Frei won the second poster prize at the first international SystemsX.ch conference in Basel for his poster entitled "Caught in the Act: Ligand-based Receptor Capturing (LRC) on Living Cells".

March 2012

"A Cell Surfaceome Map for Immunophenotyping and Sorting Pluripotent Stem Cells" manuscript published in Molecular and Cellular Proteomics

Andreas Frei won the Career Development Award 2012 at the 8th Annual US HUPO Conference "The Future of Proteomics" in San Francisco for his presentation entitled "A chemoproteomic technology for the ligand-based receptor identification on living cells and tissues".

"Comprehensive description of the N-glycoproteome of mouse pancreatic β-cells and human islets" published in Journal of Proteome Research

Andreas Frei won a Travel fellowship in order to attend US HUPO Conference "The Future of Proteomics" in San Francisco

February 2012

"Identification of new interacting partners for atypical Rho GTPases: a SILAC-based approach" manuscript published in Methods in Molecular Biology

"Proteomic surfaceome analysis of mesothelioma" manuscript published in Lung Cancer

January 2012

"CD proteome and beyond - technologies for targeting the immune cell surfaceome" review published in Frontiers of Biosciences

December 2011

Lisa Henning successfully finished her master thesis entitled "Ligand-Based Receptor Capturing for the Discovery of Drug Targets" and joined the group of
Prof. Christian Freund at the Freie Universitaet Berlin as a PhD student. Congratulations!

November 2011

Waltraud Mair, our former master student, won the Willi-Studer-Prize as the best ETH student of the biology master program. Congrats! Waltraud is now a PhD student working in Hanno Steen's lab at Harvard in collaboration with Bernhard Kuester in Munich.

The Wollscheid lab was selected for a two year Roche Postdoc Fellowship award in the context of a collaboration on infectious diseases.

Thomas Bock successfully defended his PhD thesis entitled "Proteomic surfaceome charateriztion of brain cancer". He is going to join the groups of Peer Borg and Martin Beck at EMBL in Heidelberg as an EMBL Interdisciplinary Postdocs (EIPOD) Postdoctoral fellow.

We welcome Nadine Sobotzki and Ulrich Omasits, who started their PhD in the Wollscheid group. Nadine will work on the project funded by the SINERGIA grant and Ulrich will work in the interface between the Ahrens group and the Wollscheid group.

October 2011

We congratulate Andreas Frei for winning the 2nd poster prize at the SystemsX.ch conference on Systems Biology.

We welcome Sebastian Müller, who started his PhD in the interface between the Wolfrum and the Wollscheid group.

Joanna Kopecka, a PhD student from the University of Turin, Italy, is visiting our lab for one month for using the CSC technology to quantitatively investigate the surfaceome of multi drug resistent colon cancer cells.

Proteomics Focus Issue "Focus on Stem Cell Proteomics" published in "Proteomics"

September 2011

Lisa Henning successfully finished her master thesis entitled "Ligand-based Receptor Capturing (LRC) for the Discovery of Drug Targets". Congratulations!

Our US Patent 7,879,799 "Methods for Characterizing Glycoproteins and Generating antibodies for Same" has now been publicly issued.

We congratulate Nikiana Simigdala for winning the 3rd Poster Prize at the conference of the Human Proteome Organisation (HUPO) 2011 for her poster entitled "Spatial proteomics on the Drosophila wing imaginal disc".

Jirina Tycklekova's abstract was selected for oral presentation at the 5th Central and Eastern European Proteomic Conference (CEEPC).

August 2011

Dr. Maren Depke, a guest scientist from the University of Greifswald, joined the Wollscheid lab for the next four month in order to extend her knowledge on mass spectrometry, specially MRM.

We congratulate Ferdinando Cerciello for the acceptance of the manuscript "Proteomic Surfaceome Analysis of Mesothelioma" by Annemarie Ziegler and Ferdinando Cerciello et al. by Lung Cancer.

Andreas Frei and Karel Novy both successfully applied for a travel grant from the molecular life science (MLS) PhD program. This allowed Andreas to attend the conference of the American Society for Mass Spectrometry (ASMS) in Denver and Karel to attend the conference of the Human Proteome Organisation (HUPO) in Geneva.

Juli 2011

The Wollscheid lab successfully applied for a SNF Sinergia grant together with Prof. Shana Shurla from the Food and Nutrition Toxicology Laboratory (ETHZ) and PD Dr. Giancarlo Marra from the Institute of Molecular Cancer Research (UniZH).

We congratulate Thomas Bock for receiving a travel award from the ( ZNZ) for participating at the conference of the Human Proteome Organisation (HUPO) in september 2011 in Geneva.

June 2011

Erika Meneses Romero, a scientist from the group of Dr. Cesar Ferreira Batista at the Instituto de Biotenologia, UNAM in Mexico, is visiting our lab for two month in order to improve her mass spectrometric skills.

Andreas Frei and Hansjörg Möst gave excellent talks at the conference of the American Society for Mass Spectrometry (ASMS) in Denver and got exciting feedback.

April 2011

The Wollscheid lab successfully applied for a Swiss National Foundation (SNF) grant with a proposal entitled "Ligand-based identification of receptors on living cells using Surface Receptor Capturing".

Annalisa Nicastri joined the Wollscheid lab as a guest student for the next 9 month. She will work together with Ferdinando Cerciello on the mesothelioma project.

Jirina Tyleckova retourned to her lab in the Czech Republic. We thank her for the work accomplished here.

We congratulate Damaris Bausch-Fluck for winning the first poster prize at the Proteomic Forum 2011 in Berlin for her poster about the Cell Surface Protein Atlas.

March 2011

We congratulate Andreas Frei and Hansjörg Möst for being selected to give a talk at the conference of the American Society for Mass Spectrometry (ASMS) in Denver.
The new and improved InfectX website is now online, with many information about the project and the research groups involved: www.infectx.ch

February 2011

Lisa Henning, a master student from the program "Interdisciplenary Sciences" from the ETH Zurich, joined our lab and started her master thesis under the supervision of Andreas Frei focusing on ligand-receptor interactions.

January 2011

Lydia Bänziger successfully finished her master thesis entitled "Quantitative Analysis of the Endothelial Cell Surface Proteome upon Perturbation by Cilengitide" and joined Bellevue Asset Management as Research Analyst. Congratulations!

December 2010

We congratulate Dr. med. Ferdinando Cerciello for receiving a grant from the Huggenberger-Bischoff Stiftung zur Krebsforschung.

November 2010

Ulrich Omasits, who recently finished his master thesis in the Wollscheid lab, started working in the group of Jyoti Choudhary at the Wellcome Trust Sanger Institute.

October 2010

Karel Novy started as a PhD student in the Wollscheid Lab! His initial research will focus on surfaceome biology of infection within the InfectX project (Systems Biology of pathogen entry into human cells).

September 2010

The spotlight about the Cell Surface Capturing technology has been featured in Best of Chemical Biology in 2009.

August 2010

Andreas Hofmann successfully defended his PHD thesis entitled "Proteomic Cell Surface Phenotyping of Hematological Malignancies" and is going to join Novartis as a groupleader.
Ulrich Omasits successfully finished his master thesis entitled "Bioinformatic and Mass Spectrometric Analysis of the Cell Surface Proteome" in collaboration with the University of Vienna.
Andrea Jacobs re-joined the lab as a Miltenyi Biotec fellow after her maternity leave.

July 2010

Andreas Frei was awarded with a travel fellowship from the Swiss Proteomics Society (SPS) for HUPO conference 2010 in Sydney

Waltraud Mair successfully finished her Master thesis entitled "Influenza Virosome induced changes of the HeLa surface proteome" and left for an 8 month research exchange program to Sao Paulo, Brazil

Jirina Tyleckova from the Academy of Science, Libechov, CZ, will join our lab in November for 5 month for a exchange research visit.

June 2010

Ulrich Omasits won a fellowship to attend the Computational Biology Summer School

The research of the Wollscheid lab was highlighted in the Miltenyi Biotec newsletter MACS&more Special edition neuroscience MACS&more Special edition neuroscience [pdf]

May 2010 - Research exchange visit

Anetta Härtlova from the University of Defense, Hradec Králové, CZ, will join our lab for a research exchange.

Dec 2009 - Poster Prize

Thomas Bock (Wollscheid Group) won the Poster Prize at the Swiss Proteomics Society Congress 2009 (SPS) in the category "Systems Biology" for his poster ""Protein Dynamics: Protein Quantification in Time and Space" about the Glioma Cell Surface Signal Transduction Network. link

June 2009 - Highlights in Nature Methods and ACS Chemical Biology

The recently published article about the Cell Surface Capturing Technology has been highlighted in Nature Methods and ACS Chemical Biology. [link to Nat Method article / pdf download], [link to article in ACS Chem Biol]

May 2009 - Press release of ISB

The Institute for Systems Biology in Seattle emphasizes in their press release the recently published CSC Technology in Nature Biotechnology. [link]