Regulatory protein-metabolite interactions

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Regulatory interactions between metabolites and regulatory proteins (eg transcription factors and kinases) or enzymes trigger and control cell responses, yet is largely a terra incognita. Increasing evidence suggests that the majority of proteins bind specific metabolites and that such interactions are relevant to metabolic and gene regulation. In sharp contrast to physical mapping of protein-protein or protein-DNA interactions, there are no methods to systematically identify functional allosteric protein- metabolite interactions.

In various projects, we pursue a combined experimental (often using dynamic metabolomics data) and computational approach to discover in vivo relevance of metabolite-protein interactions and to identify their specific role in the larger regulation scheme. A particular focus is on E. coli and B. subtilis central metabolism and TORC1 signaling in yeast.

A systems biology view of metabolism and its regulation
A systems biology view of metabolism and its regulation (from Gerosa and Sauer, Curr Opin Biotech 2011)


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