Metabolomics

Main content

While fluxes are the functional output of metabolism, intracellular metabolite concentrations are its key constituents. In collaboration with the Zamboni lab, we develop metabolomics methods on several high-end mass spectrometers. In collaboration with Agilent Inc. we focus currently on applications of our novel high-throughput metabolomics method that quantifies 500-1000 metabolites in a given sample per minute. Our latest advance is the ability to measure intracellular metabolites automatically in real-time. With the analytical problems of data generation largely solved, the current focus is on computational data analysis and biological hypothesis generation. Several large-scale metabolomics projects, including the entire E. coli gene deletion library, are currently being finalized.

Metabolites identified in mouse liver tissue by our TOF-MS method
Metabolites identified in mouse liver tissue by our TOF-MS method

References

  • Link H, Fuhrer T, Gerosa L, Zamboni N, and Sauer U (2015). Real-time metabolome profiling of the metabolic switch between starvation and growth. Nat. Methods, vol. 12, no. 11, pp. 1091–1097
  • Sevin DC & Sauer U. Ubiquinone accumulation improves osmotic stress tolerance in E. coli. Nature Chem Biol. 2014 10:266-272 Pubmed
  • Zimmermann M, Sauer U, Zamboni N. Quantification and mass isotopomer profiling of α-keto acids in central carbon metabolism. Anal Chem 2014; 86(6):3232-7 Pubmed
  • Heux S, Fuchs T, Buhmann J, Zamboni N & Sauer U. A high-throughput metabolomics method to predict high concentration cytotoxicity of drugs from low concentration profiles. Metabolomics 8:433-443.
  • Fendt SM, Büscher JM, Rudroff F, Picotti P, Zamboni N & Sauer U. Tradeoff between enzyme and metabolite efficiency maintains metabolic homeostasis upon perturbations in enzyme capacity. Mol. Sys. Biol. 2010; 13;6:356. Pubmed

 

 

 
 
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