Zrinka Raguz Nakic
Phosphorylation is an important post-translational protein modification known to affect the active protein pool in several ways. Although many phosphorylation sites have been postulated in a variety of organisms, functionality has been demonstrated only for very few of them.
In my PhD project, I am aiming to identify functional phosphorylation sites in a high throughput manner by applying a new approach. Specifically, I first identify good candidate phosphorylation sites and perform mutagenesis of these candidates. In order to assign functionality to the sites, I use high-throughput untargeted mass spectrometry to measure the metabolome of the mutants, and compare the mutant profiles to the wild type.
Student projects are available!