Dr. Karl Kochanowski
I studied Biology at RWTH Aachen in Germany. During my diploma thesis, I worked on the identification of a metabolic flux sensor in Escherichia coli in the lab of Matthias Heinemann at ETH Zurich. In May 2010, I joined the lab of Prof. Uwe Sauer as a PhD student.
In my PhD project, I aim at elucidating the role of dynamic protein-metabolite interactions in metabolic adaptation processes of Escherichia coli: which protein-metabolite interactions are relevant in vivo, and when? Towards this end, I combine experimental methods such as metabolomics and fluorescent transcriptional reporters with computational approaches such as kinetic modeling.
Several student projects are currently available.
Zavrel, M., Kochanowski, K. & Spiess, A. C. Comparison of different approaches and computer programs for progress curve analysis of enzyme kinetics. Eng. Life Sci. 10, 191–200 (2010)
Kochanowski, K., Volkmer, B., Gerosa, L., Haverkorn van Rijsewijk, BR., Schmidt, A. & Heinemann, M. Functioning of a metabolic flux sensor in Escherichia coli. PNAS 110, 1130–5 (2013)
Link, H., Kochanowski, K. & Sauer, U. Systematic identification of allosteric protein-metabolite interactions that control enzyme activity in vivo. Nat. Biotechnol. 31, 357–61 (2013)
Gerosa, L.*, Kochanowski, K.*, Heinemann, M. & Sauer, U. Dissecting specific and global transcriptional regulation of bacterial gene expression. Mol. Syst. Biol. 9, 658 (2013)
Kochanowski, K., Sauer, U. & Chubukov, V. Somewhat in control-the role of transcription in regulating microbial metabolic fluxes. Curr. Opin. Biotechnol. 1–7 (2013)
Chubukov, V.*, Gerosa, L.*, Kochanowski, K.* & Sauer, U. Coordination of microbial metabolism. Nat. Rev. Microbiol. 12, 327–40 (2014)