Aebersold Group

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Aebersold Group 2012

Quantitative Mass Spectrometry

The Aebersold group is interested in developing and applying novel methods in quantitative mass spectrometry to accurately measure protein analytes in complex samples.

The team of Prof. Ruedi Aebersold employs computational and experimental approaches to accurately quantify protein abundance and protein activity across many different biological samples. To achieve this, the Aebersold lab applies and extends existing high throughput proteomics methods such as labelfree shotgun proteomics and targeted proteomics using SRM (or SWATH-MS). In order to gain deeper insight into protein interactions, techniques using affinity purification and chemical cross-linking are employed in the lab. Read more

 

 

Group News

 

06.12.2016

Epigenetic stress responses induce muscle stem-cell ageing by Hoxa9 developmental signals

Histone modification profiling in rare stem cell populations: our collaborative work on the epigenetics in muscle stem cell aging got published in Nature! Read more 

03.12.2016

Aebersold lab: Master thesis/semester project in quantitative AP-MS of signaling complexes

TNF-alpha is a cytokine playing a fundamental role in inflammation that induces the formation of a large signaling complex associated with its receptor. The project will entail optimization of AP-MS workflow applied to this complex. The student will learn protein purification, experimental and computational aspects of AP-MS and possibly other proteomics techniques. The student is expected to know basics of mass spectrometry and cell culture and have a strong interest in method development. Read more 

03.12.2016

Aebersold lab: Master Thesis in Population Genetics—Analyzing Proteomics in a Diverse Population to Uncover Mechanisms of Aging

The process of aging can be better understood from a systems biology approach—examining how entire cellular pathways change across time. This project involves generating then analyzing transcriptomic, proteomic, and metabolomic data from liver tissue of 660 individual mice from different ages, which also segregate by genetics and diet. Project keywords: genetics, aging, multi-omics, metabolism, bioinformatics, statistics, systems biology, big data, personalized medicine. Read more 

07.10.2016

Postdoc Project Quantitative proteomic and phosphoproteomic analyses of the PD-1 co-inhibitory pathways in primary effector T cells

Project aims to provide a system-level view of the mechanisms of action of PD-1, a key T cell co-inhibitor in cancer. In the project, signalosome dynamics in primary effector T cells will be analyzed with high-throughput quantitative proteomics and phosphoproteomics. The method to be developed is scalable and will be applicable to the analysis of the mode of action of “checkpoint inhibitors” under physiological and pathological conditions. Read more 

04.08.2016

Aebersold group: Master Thesis of systems biology to reveal the protein turnover behaviors in human cancer cells

The student will learn how to efficiently process samples for mass spectrometry analysis at a large scale, how to perform and maintain a mass spectrometer for their own samples in SWATH-MS mode, and how to analyze the data generated using systems biology perspectives. Read more 

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08.12.2016
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