Aebersold lab: Master thesis/semester project in quantitative AP-MS of signalling complexes
TNF-alpha is a cytokine playing a central role in inflammation that induces the formation of a large signaling complex associated with its receptor. The project will primarily entail the application of quantitative mass spectrometry methods to obtain detailed information about this large assembly. The student will learn protein purification, experimental and computational aspects of AP-MS, and is expected to know basics of mass spectrometry and cell culture.
Tumor necrosis factor-alpha (TNF-alpha) is an extensively studied cytokine that plays a fundamental role in inflammatory processes. Binding of TNF-alpha to TNFRI leads to the formation of a large signaling complexes consisting of about 30 proteins (TNF-RSC). The project will primarily entail method optimization of the current AP-MS (affinity purification coupled to mass spectrometry) workflow as applied to the TNF-RSC to improve its throughput and specificity as well as to obtain both quantitative and structural information about this large assembly.
Dr. Rodolfo Ciuffa and Federico Uliana will jointly supervise the project. Under their supervision, the student will have the opportunity to learn protein purification, experimental and computational aspects of AP-MS and possibly advanced quantitative and structural proteomics techniques. The student is expected to have basic knowledge of mass spectrometry, training in cell culture and a strong interest in method development.
For further information, please send a CV and a motivation statement to Dr. Rodolfo Ciuffa () and Federico Uliana ().
 Brenner, D.; Blaser, H.; Mak, T. W. Regulation of tumour necrosis factor signalling: live or let die. Nat. Rev. Immunol. 2015, 15 (6), 362−374.