Sauer group: Metabolomics and Flux Balance Analysis in Saccharomyces cerevisiae (Semester/Master Project)

Background

In order to function optimally in diverse environments, cells need to regulate their metabolic fluxes in response to changes in nutrient availability. We are combining detailed physiological measurements with mathematical models of metabolism to generate hypotheses about the regulation of metabolism via enzyme phosphorylation.

This project represents an opportunity for you to perform a systems biology research project that involves growing yeast cultures, measuring samples with high-throughput enzyme assays and state-of-the-art metabolomics technology, and analyzing data using genome-scale flux balance analysis.

This project will involve approximately 70% wet-lab and 30% computational work. Proficiency with MATLAB/python is beneficial but not required.

If you are interested, please contact Brendan Ryback ()

Literature

Oliveira et al., Regulation of yeast central metabolism by enzyme phosphorylation, Mol Syst Biol. 2012.