Aebersold Lab, Proteomics, Method development in the field of phosphoproteomics

Master thesis or research projects available

by Nicola Zamboni

Protein phosphorylation is one the most important post-translational modifications with relevance for many biological processes, e.g. signal transduction. Phosphoproteomics aims to comprehensively characterize this modification by mass spectrometry. To be able to detect phosphorylation events, proteins are enzymatically digested and phosphopeptides enriched by various affinity materials. Our group has a strong track record in developing phosphoproteomics methods and applying them to highly relevant research questions in systems biology and biomedicine.

Currently, we are evaluating different affinity materials based on metal oxides for the analysis of small sample amounts and are looking for students to support us in this endeavor.

You will learn how to perform phosphopeptide enrichment, process your samples for MS analysis, analyze the samples by LC-MS and perform the data analysis. These projects are the perfect opportunity to get hands-on experience with proteomics methods and mass spectrometry instrumentation. You will contribute to important projects that will have immediate impact on cutting-edge research in the group. Prior experience with mass spectrometry is not essential, although candidates with basic knowledge of proteomics methods are preferred.

For further information, contact Dr. Alexander Leitner ().

Thematic background:

Jünger M. A., Aebersold R., Mass spectrometry-driven phosphoproteomics: Patterning the systems biology mosaic, WIREs Dev. Biol., 3 (2014) 83-112.

Link: external pagehttp://onlinelibrary.wiley.com/doi/10.1002/wdev.121/abstract

Recent applications:

Vaga S. et al., Phosphoproteomic analyses reveal novel cross-modulation mechanisms between two signaling pathways in yeast, Mol. Syst. Biol., 10 (2014) 767.

Link: external pagehttp://msb.embopress.org/content/10/12/767

Oliveira et al., Dynamic phosphoproteomics reveals TORC1-dependent regulation of yeast nucleotide and amino acid biosynthesis, Sci. Signal., 8 (2015) rs4.

Link: external pagehttp://stke.sciencemag.org/content/8/374/rs4

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