Discovery of Glycoprotein Biomarkers for Aggressive Prostate Cancer
Researchers from the Aebersold group used SWATH mass spectrometry to profile the glycoproteome extracted from clinical tissues for the discovery of aggressive prostate cancer biomarkers.
The identification of biomarkers indicating the level of aggressiveness of prostate cancer (PCa) will address the urgent clinical need to minimize the general over-treatment of patients with non-aggressive PCa, which account for the majority of PCa cases. Here, the IMSB researchers, together with their collaborator in Johns Hopkins University, isolated formerly N-linked glycopeptides from normal prostate, non-aggressive, aggressive and metastatic PCa tumor tissues and analyzed the samples by SWATH mass spectrometry, an emerging data independent acquisition method that generates a single file containing fragment ion spectra of all ionized species of a sample. Glycoproteins showing significant quantitative changes associated with diverse biological processes involved in PCa aggressiveness and metastasis were revealed and verified, pointing out potential new tissue biomarkers to avoid overtreatment of non-aggressive PCa.
Reference: Liu Y, Chen J, Sethi A, Li QK, Chen L, Collins B, Gillet LC, Wollscheid B, Zhang H, Aebersold R. Glycoproteomic Analysis of Prostate Cancer Tissues by SWATH Mass Spectrometry Discovers N-acylethanolamine Acid Amidase and Protein Tyrosine Kinase 7 as Signatures for Tumor Aggressiveness. Mol Cell Proteomics. 2014 Jul;13(7):1753-68. external pageDOI